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Phlebotomy NHA-CPT | 200+ Questions on Tube Additives, Hemolysis, Urine Testing & Patient Safety | NHA Certification 2025/2026

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This document presents over 200 structured exam-style questions and answers built for the NHA-CPT (Certified Phlebotomy Technician) exam as administered by the National Healthcareer Association (NHA) for the 2025/2026 academic year. The content blends detailed clinical definitions with testing cues and real-world applications to reinforce mastery of essential phlebotomy procedures, lab safety, patient care, and diagnostic knowledge. Topics covered include: Tube top colors and additives: EDTA, sodium citrate, heparin, fluoride, clot activators, and light-sensitive amber tubes Order of draw and tube selection for specific tests like A1c, ABG, CBC, GTT, and urinalysis Hemolysis causes and prevention: improper syringe use, extreme temperatures, and sample handling errors Urine sample protocols, including clean catch methods, 24-hour collections, and preservation temperatures Semen analysis procedures and abstinence requirements Patient populations and considerations: geriatrics, pediatrics, bariatric patients, and arterial site risks Standard precautions, OSHA compliance, MSDS awareness, and exposure management Blood vessel anatomy, venipuncture site selection, and complications such as hematoma, phlebitis, or rolling veins HIPAA privacy standards, consent types, labeling, and communication for proper patient identification Blood smear preparation, malaria testing, iatrogenic anemia, and causes of abnormal glucose levels Ideal for: Students in Phlebotomy Technician, Medical Assisting, Pre-Nursing, Clinical Laboratory Science, or Healthcare Science programs Learners in community colleges, vocational health schools, and online NHA-CPT prep programs Instructors seeking up-to-date content for practice tests, clinical reasoning scenarios, or certification exam review sessions This document is exam-ready, practice-oriented, and structured for rapid recall—equipping students to confidently tackle both classroom evaluations and the national certification exam. Keywords: tube additives, phlebotomy exam prep, hemolysis causes, urine sample handling, semen analysis, sodium citrate, OSHA safety, ABG sample, A1c testing, hematoma prevention, pediatric phlebotomy, HIPAA compliance, light-sensitive tubes, rolling veins, NHA CPT 2025

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Uploaded on
November 29, 2025
Number of pages
7
Written in
2025/2026
Type
Exam (elaborations)
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Questions & answers

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Phlebotomy NHA 2025/2026 Exam
Questions and Correct Answers | New
Update



EDTA - 🧠 ANSWER ✔✔Ethylenediaminetetraacete. A calcium chelating

(binding) agent that is used as an anticoagulant for laboratory blood

specimens


Hazard for phlebotomist - 🧠 ANSWER ✔✔Needlesticks


Post Vasectomy Semen Analysis - 🧠 ANSWER ✔✔to confirm if sperm is

present or not.


Color of tube that protects from light - 🧠 ANSWER ✔✔Amber


NPO - 🧠 ANSWER ✔✔nothing by mouth

, Thrombocytopenia - 🧠 ANSWER ✔✔low platelet count


geriatrics - 🧠 ANSWER ✔✔the care of aging people over 65


Normal blood glucose range - 🧠 ANSWER ✔✔100-126


Stat lab time - 🧠 ANSWER ✔✔45-60 min


PPD - 🧠 ANSWER ✔✔purified protein derivative (TB test) 10mm or greater

positive


Light Blue Tube - 🧠 ANSWER ✔✔Must be filled 100percent


Diurnal Effect - 🧠 ANSWER ✔✔Monitor certain drugs


Exposed to biohazards - 🧠 ANSWER ✔✔Wash immediately with soap and

water

OSHA (Occupational Safety and Health Administration) - 🧠 ANSWER

✔✔provides guidelines for workplace safety, and enforces these

regulations.


Blood sample that has higher glucose - 🧠 ANSWER ✔✔Capillary


Fasting - 🧠 ANSWER ✔✔8-12 hours before test

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