UNCW BIO 201 LAB PRACTICAL QUESTIONS WITH
COMPLETE SOLUTIONS
Absorbance Correct Answers log(Initial Intensity of light/Light
Intensity at Sample)
Acidity Correct Answers The concentration of hydrogen ions
(H+) in a solution.
Aerobic Respiration Correct Answers Uses oxygen to harvest
energy fro organic molecules like glucose.
An Ideal Pigment Correct Answers Its absorbance should
double when its concentration is doubled. This is because
absorbance is linearly proportional to its concentration.
Animal Cell in Various Osmotic Conditions:
Isotonic
Hypertonic
Hypotonic Correct Answers No Change
Crenation
Lysis
Are the results of the effects of light intensity on reaction as you
expected? Correct Answers Yes, the more light the reaction is
exposed to the faster the rate of the reaction goes.
Arm Correct Answers Part of the microscope connecting the
body tube to the base of the microscope.
As you increase magnification, what happens to the:
,1) field of view
2) depth of focus
3) working distance Correct Answers 1) decreases
2) decreases
3) increases
Based on your observations of the iodine experiment, what can
you conclude about the selective permeability of dialysis tubing
to iodine, glucose, and starch (what can fit through the holes and
what can't)? How did you know what was able to pass through?
Correct Answers The Iodine Test tests for the presence of starch
in a sample.
Beer-Lambert Law Correct Answers A=abc. (A) is absorbance.
(a) is the absorbance coefficient which is constant for the
particular substance. (b) is the path length or the distance light
must travel through the sample. (c) is the concentration of the
pigment.
Benedict's Test Correct Answers Qualitative test to test for
reducing sugars.
Body Tube Correct Answers The part of the microscope
connecting the ocular and objective lenses.
Bradford Assay Correct Answers A quantitative determination
of protein concentration. Uses the change in color from red to
blue when the pigment Coomassie binds to a protein. The
intensity of the blue pigment is measured quantitatively using a
spectrophotometer. BSA or bovine serum albumin is used to
generate protein standards.
, Buffer Correct Answers A solution which resists changes in pH
when acids or bases are added to them. Most buffers contain
approximately equal amounts of weak acid and its related base.
Carbohydrates Correct Answers Store energy as well as provide
structural support.
Cellular Respiration Correct Answers One of the principle
metabolic processes in cells.
Chromosomes Correct Answers Structures that carry genetic
information in the nucleus of a cell.
Codon Correct Answers A sequence of three nucleotides that
together form a unit of genetic code in a DNA or RNA
molecule.
Concentration Gradient Correct Answers The flow from high
concentration to areas of low concentration.
Condenser Correct Answers Lens that focuses light from the
light source onto the specimen.
Cupric (copper) Ion Correct Answers The indicator in the
Benedict's Test to see if there is a reducing sugar. If a reducing
sugar is present then cuprous oxide is formed which has a
reddish orange color.
Cuvette Correct Answers What a solution is put in to go into the
Spectrophotometer.
COMPLETE SOLUTIONS
Absorbance Correct Answers log(Initial Intensity of light/Light
Intensity at Sample)
Acidity Correct Answers The concentration of hydrogen ions
(H+) in a solution.
Aerobic Respiration Correct Answers Uses oxygen to harvest
energy fro organic molecules like glucose.
An Ideal Pigment Correct Answers Its absorbance should
double when its concentration is doubled. This is because
absorbance is linearly proportional to its concentration.
Animal Cell in Various Osmotic Conditions:
Isotonic
Hypertonic
Hypotonic Correct Answers No Change
Crenation
Lysis
Are the results of the effects of light intensity on reaction as you
expected? Correct Answers Yes, the more light the reaction is
exposed to the faster the rate of the reaction goes.
Arm Correct Answers Part of the microscope connecting the
body tube to the base of the microscope.
As you increase magnification, what happens to the:
,1) field of view
2) depth of focus
3) working distance Correct Answers 1) decreases
2) decreases
3) increases
Based on your observations of the iodine experiment, what can
you conclude about the selective permeability of dialysis tubing
to iodine, glucose, and starch (what can fit through the holes and
what can't)? How did you know what was able to pass through?
Correct Answers The Iodine Test tests for the presence of starch
in a sample.
Beer-Lambert Law Correct Answers A=abc. (A) is absorbance.
(a) is the absorbance coefficient which is constant for the
particular substance. (b) is the path length or the distance light
must travel through the sample. (c) is the concentration of the
pigment.
Benedict's Test Correct Answers Qualitative test to test for
reducing sugars.
Body Tube Correct Answers The part of the microscope
connecting the ocular and objective lenses.
Bradford Assay Correct Answers A quantitative determination
of protein concentration. Uses the change in color from red to
blue when the pigment Coomassie binds to a protein. The
intensity of the blue pigment is measured quantitatively using a
spectrophotometer. BSA or bovine serum albumin is used to
generate protein standards.
, Buffer Correct Answers A solution which resists changes in pH
when acids or bases are added to them. Most buffers contain
approximately equal amounts of weak acid and its related base.
Carbohydrates Correct Answers Store energy as well as provide
structural support.
Cellular Respiration Correct Answers One of the principle
metabolic processes in cells.
Chromosomes Correct Answers Structures that carry genetic
information in the nucleus of a cell.
Codon Correct Answers A sequence of three nucleotides that
together form a unit of genetic code in a DNA or RNA
molecule.
Concentration Gradient Correct Answers The flow from high
concentration to areas of low concentration.
Condenser Correct Answers Lens that focuses light from the
light source onto the specimen.
Cupric (copper) Ion Correct Answers The indicator in the
Benedict's Test to see if there is a reducing sugar. If a reducing
sugar is present then cuprous oxide is formed which has a
reddish orange color.
Cuvette Correct Answers What a solution is put in to go into the
Spectrophotometer.
