BRAINSCAPE1
Biotech bace Exam with complete
solutions latest version
Which of the following is a product of agricultural biotechnology? - CORRECT
ANSWER-Transgenic crops
Which of the following magnification levels would be best for viewing bacterial
organelles using an oil immersion lens? - CORRECT ANSWER-1,000X
Gram staining can determine which bacterial characteristic? - CORRECT ANSWER-
Peptidoglycan content
Why must technicians practice aseptic technique when culturing cells? - CORRECT
ANSWER-To ensure they are growing pure cultures of their desired cells.
Which of the following techniques destroys all microorganisms? - CORRECT ANSWER-
Sterilization
A pH meter electrode measures pH by measuring the relative concentration of which of
the following? - CORRECT ANSWER-Hydrogen ion
Which of the following is typically measured using a spectrophometer set at a
wavelength of 600 nm? - CORRECT ANSWER-The concentration of bacteria in a
suspension
According to the Beer-Lambert law, which of the following factors does NOT affect the
absorbance of a solution? - CORRECT ANSWER-Temperature of the solution
Salt (such as sodium chloride or sodium acetate) is commonly used in procedures to
extract plasmid DNA. Which of the following best describes the role of the salt? -
CORRECT ANSWER-The positively charged sodium ions neutralize the negative
charge on the DNA.
When using an acrylamide gel for protein electrophoresis, it is best to use a higher
percentage of acrylamide to resolve smaller proteins, while large proteins are best
resolved on low-percent gels. - CORRECT ANSWER-True
BRAINSCAPE1
, BRAINSCAPE1
1. Staining with Coomassie Blue
2. Adding loading dye to protein samples
3. Adding a molecular weight standard to an empty well
4. Adding SDS to the sample buffer and the PAGE running buffer - CORRECT
ANSWER-1. To visualize proteins after PAGE
2. To facilitate loading samples into wells, and to monitoring sample migration during
PAGE
3. To determine the relative sizes of the samples run on the gel
4. To coat the sample proteins with a negative charge, and denature them
A restriction enzyme digestion reaction contains which of the following reagents? -
CORRECT ANSWER-DNA, Water, Buffer, Restriction Enzyme
A plasmid has three restriction sites for EcoRI. If the plasmid is treated with EcoR1, how
many fragments will be obtained? - CORRECT ANSWER-3
steps of a general protocol for genetic engineering - CORRECT ANSWER-1. Isolate
gene of interest
2. Paste/clone gene of interest into a vector
3. Transform host cells with vector
4. Grow recombinant cells
Which of the following techniques should be used to amplify specific DNA sequences? -
CORRECT ANSWER-PCR
the sequence of a PCR cycle - CORRECT ANSWER-1. The mixture is heated to
denature the double stranded DNA.
2. The primers hybridize to the target DNA.
3. DNA polymerase extends the primers to make a copy of the target DNA.
What is the primary advantage of using Escherichia coli for protein expression studies?
- CORRECT ANSWER-It can produce high yields of protein quickly and is easy to
manipulate genetically.
Many biopharmaceuticals must go through a purification process to separate them from
cellular debris, media, and other components used during cell culture. Which of the
following is a commonly used technique to separate proteins from complex mixtures? -
CORRECT ANSWER-Chromatography
A technician is asked to verify the presence of a protein of interest after a production
run. Following purification, a sample is run on an SDS-PAGE gel, and a band appears
at the expected molecular weight. Which of the following techniques is the best method
to confirm that the band represents the purified protein of interest? - CORRECT
ANSWER-Run a western blot with a sample of the fraction
BRAINSCAPE1
Biotech bace Exam with complete
solutions latest version
Which of the following is a product of agricultural biotechnology? - CORRECT
ANSWER-Transgenic crops
Which of the following magnification levels would be best for viewing bacterial
organelles using an oil immersion lens? - CORRECT ANSWER-1,000X
Gram staining can determine which bacterial characteristic? - CORRECT ANSWER-
Peptidoglycan content
Why must technicians practice aseptic technique when culturing cells? - CORRECT
ANSWER-To ensure they are growing pure cultures of their desired cells.
Which of the following techniques destroys all microorganisms? - CORRECT ANSWER-
Sterilization
A pH meter electrode measures pH by measuring the relative concentration of which of
the following? - CORRECT ANSWER-Hydrogen ion
Which of the following is typically measured using a spectrophometer set at a
wavelength of 600 nm? - CORRECT ANSWER-The concentration of bacteria in a
suspension
According to the Beer-Lambert law, which of the following factors does NOT affect the
absorbance of a solution? - CORRECT ANSWER-Temperature of the solution
Salt (such as sodium chloride or sodium acetate) is commonly used in procedures to
extract plasmid DNA. Which of the following best describes the role of the salt? -
CORRECT ANSWER-The positively charged sodium ions neutralize the negative
charge on the DNA.
When using an acrylamide gel for protein electrophoresis, it is best to use a higher
percentage of acrylamide to resolve smaller proteins, while large proteins are best
resolved on low-percent gels. - CORRECT ANSWER-True
BRAINSCAPE1
, BRAINSCAPE1
1. Staining with Coomassie Blue
2. Adding loading dye to protein samples
3. Adding a molecular weight standard to an empty well
4. Adding SDS to the sample buffer and the PAGE running buffer - CORRECT
ANSWER-1. To visualize proteins after PAGE
2. To facilitate loading samples into wells, and to monitoring sample migration during
PAGE
3. To determine the relative sizes of the samples run on the gel
4. To coat the sample proteins with a negative charge, and denature them
A restriction enzyme digestion reaction contains which of the following reagents? -
CORRECT ANSWER-DNA, Water, Buffer, Restriction Enzyme
A plasmid has three restriction sites for EcoRI. If the plasmid is treated with EcoR1, how
many fragments will be obtained? - CORRECT ANSWER-3
steps of a general protocol for genetic engineering - CORRECT ANSWER-1. Isolate
gene of interest
2. Paste/clone gene of interest into a vector
3. Transform host cells with vector
4. Grow recombinant cells
Which of the following techniques should be used to amplify specific DNA sequences? -
CORRECT ANSWER-PCR
the sequence of a PCR cycle - CORRECT ANSWER-1. The mixture is heated to
denature the double stranded DNA.
2. The primers hybridize to the target DNA.
3. DNA polymerase extends the primers to make a copy of the target DNA.
What is the primary advantage of using Escherichia coli for protein expression studies?
- CORRECT ANSWER-It can produce high yields of protein quickly and is easy to
manipulate genetically.
Many biopharmaceuticals must go through a purification process to separate them from
cellular debris, media, and other components used during cell culture. Which of the
following is a commonly used technique to separate proteins from complex mixtures? -
CORRECT ANSWER-Chromatography
A technician is asked to verify the presence of a protein of interest after a production
run. Following purification, a sample is run on an SDS-PAGE gel, and a band appears
at the expected molecular weight. Which of the following techniques is the best method
to confirm that the band represents the purified protein of interest? - CORRECT
ANSWER-Run a western blot with a sample of the fraction
BRAINSCAPE1
