ASCP CG Exam (Classic
Cytogenetics)
A proper banding sample may be anticipated while we take a look at chromosomes before
banding underneath the section scope with the following look: - ANS-Dark.
A PB sample is clotted. What do you do? - ANS-Break up the clot.
About how long does mitosis final? - ANS-About 15mins.
Adding the drug Colcemid to mobile cultures can purpose all of the following phenomenons to
occur besides: - ANS-Cell swelling.
All of the following have buffering capability besides: - ANS-L-Glutamine.
All of the subsequent statistics is required during the specimen installation process besides: -
ANS-Infectious agent.
All of the following will result in tradition failure except: - ANS-Transport at room temp.
At 24hrs of publicity to PHA, what's going to be taking place to T-lymphocytes? - ANS-Increase
in RNA synthesis, signaling a transformation of lymphocytes to lymphoblasts.
At 48hrs of publicity to PHA, what will be taking place to T-lymphocytes? - ANS-Most of the
mitoses determined are the end result of 1 mobile department.
At 72hrs of publicity to PHA, what's going to be occurring to T-lymphocytes? - ANS-Most cells
have undergone 2 cellular divisions.
At 96hrs of exposure to PHA, what's going to be going on to T-lymphocytes? - ANS-Cells have
exhausted media and start to die.
Describe the physical differences among villi and decidua. - ANS-The villi might be branched in
appearance and lighter in comparison to the decidua, with a view to be extra sheet-like and
darker.
Does advantageous or negative heterpyknoesis stain dark? - ANS-Positive.
During slidemaking all of the following elements are crucial besides for: - ANS-Slides are 1st
being dipped in acetic acid.
,For how lengthy can cells be stored in -ninety ranges C? - ANS-For as much as a year.
For stable stains, how can we sequentially stain? - ANS-Q-banding and AgNOR; G-banding and
FISH.
Hoe does the culture media seem while a contamination has occurred? - ANS-Cloudy and
turbid.
How are CVS samples retrieved? - ANS-Transcervically and transabdominally.
How are percutaneous umbilical blood samples (PUBS) retrieved? - ANS-Transabdominally with
the help of ultrasonography.
How are slides aged at RT? - ANS-Airtight for 3 days to 6 weeks.
How can we chemically age slides? - ANS-Treat with 15% H2O2 (2-7mins) previous to banding.
How do clastogens have an effect on the DNA restore gadget in normal and strange cells? -
ANS-Normal cells stricken by clastogens might be capable of repair most of the damage
triggered, whereas the atypical cells will no longer.
How do slides want to be saved? - ANS-In an airtight box with temp and humidity manage.
How do we verify a mycoplasma contamination? - ANS-By staining with DAPI and Hoechst
33258.
How do we dehydrate a slide? - ANS-By exposing the slide to ethanol mixtures in the opposite
route of hydration.
How will we denature DNA for FISH? - ANS-We treat in 70% formamide/2xSSC at 70 levels C.
How can we decorate spreading? - ANS-By dropping on wet slides and losing at a forty five
degree attitude from top to backside.
How do we hybridize DNA in FISH? - ANS-The probe is hybridized onto slides at 37 stages C
for 30mins to 16hrs depending on probe used.
How do we hydrate/rehydrate a slide? - ANS-By exposing it to diverse dilutions of ethanol
shifting from a low percent of water to a better percentage of water; one
hundred%-ninety%-80%-70%-EtOH.
How do we know if a sample is amniotic fluid or maternal urine? - ANS-Normal AF is straw
coloured and passes the Fern Test.
, How can we recognize that the slides are properly wiped clean? - ANS-They retain, in
preference to repel, water while dipped.
How do we lessen cell contamination of a chorionic villus pattern? - ANS-By casting off the
adherent maternal decidua.
How can we stimulate the increase of neoplastic cells or B-cells neoplasms? - ANS-Run one
unstimulated subculture and one with a B-cell mitogen.
How can we save a bone marrow sample if it cannot be cultured inside 4 hours? - ANS-Add to
media and store at room temp.
How can we store fixed mobile pellets? - ANS-At -20 degrees C for 2-5yrs in fixative.
How do we check for chromosome breakage syndromes? - ANS-By including clastogens.
How can we troubleshoot slides drying too speedy, without adjusting the temperature or
humidity of the thermotron? - ANS-Methanol may be delivered to the fix or bloodless slides may
be used.
How do we troubleshoot slides drying too slowly with out adjusting the temperature or humidity
of the thermotron? - ANS-Warm slides may be used or airflow across the slides may be
improved.
How do you keep away from cell contamination of prenatal specimens? - ANS-Discard the first
1-2 cc of amniotic fluid drawn.
How do you keep away from mobile infection of stable tumor specimens? - ANS-Dissect away
normal parenchyma and fatty tissue.
How do you keep away from microbial contamination? - ANS-By using aseptic approach, sterile
boxes and antibiotics/fungicides.
How do you correct a bacterial or fungal infection of a pattern? - ANS-Add antibiotic to series
media or soak/wash sample in answer containing antibiotic.
How do you accurate clotting in BM specimens? - ANS-Break up clot robotically or location in 37
degree water tub.
How do you accurate slow increase in a sample that changed into amassed in EDTA vs. Sodium
heparin? - ANS-Wash the cells before setting tradition or request every other pattern.
Cytogenetics)
A proper banding sample may be anticipated while we take a look at chromosomes before
banding underneath the section scope with the following look: - ANS-Dark.
A PB sample is clotted. What do you do? - ANS-Break up the clot.
About how long does mitosis final? - ANS-About 15mins.
Adding the drug Colcemid to mobile cultures can purpose all of the following phenomenons to
occur besides: - ANS-Cell swelling.
All of the following have buffering capability besides: - ANS-L-Glutamine.
All of the subsequent statistics is required during the specimen installation process besides: -
ANS-Infectious agent.
All of the following will result in tradition failure except: - ANS-Transport at room temp.
At 24hrs of publicity to PHA, what's going to be taking place to T-lymphocytes? - ANS-Increase
in RNA synthesis, signaling a transformation of lymphocytes to lymphoblasts.
At 48hrs of publicity to PHA, what will be taking place to T-lymphocytes? - ANS-Most of the
mitoses determined are the end result of 1 mobile department.
At 72hrs of publicity to PHA, what's going to be occurring to T-lymphocytes? - ANS-Most cells
have undergone 2 cellular divisions.
At 96hrs of exposure to PHA, what's going to be going on to T-lymphocytes? - ANS-Cells have
exhausted media and start to die.
Describe the physical differences among villi and decidua. - ANS-The villi might be branched in
appearance and lighter in comparison to the decidua, with a view to be extra sheet-like and
darker.
Does advantageous or negative heterpyknoesis stain dark? - ANS-Positive.
During slidemaking all of the following elements are crucial besides for: - ANS-Slides are 1st
being dipped in acetic acid.
,For how lengthy can cells be stored in -ninety ranges C? - ANS-For as much as a year.
For stable stains, how can we sequentially stain? - ANS-Q-banding and AgNOR; G-banding and
FISH.
Hoe does the culture media seem while a contamination has occurred? - ANS-Cloudy and
turbid.
How are CVS samples retrieved? - ANS-Transcervically and transabdominally.
How are percutaneous umbilical blood samples (PUBS) retrieved? - ANS-Transabdominally with
the help of ultrasonography.
How are slides aged at RT? - ANS-Airtight for 3 days to 6 weeks.
How can we chemically age slides? - ANS-Treat with 15% H2O2 (2-7mins) previous to banding.
How do clastogens have an effect on the DNA restore gadget in normal and strange cells? -
ANS-Normal cells stricken by clastogens might be capable of repair most of the damage
triggered, whereas the atypical cells will no longer.
How do slides want to be saved? - ANS-In an airtight box with temp and humidity manage.
How do we verify a mycoplasma contamination? - ANS-By staining with DAPI and Hoechst
33258.
How do we dehydrate a slide? - ANS-By exposing the slide to ethanol mixtures in the opposite
route of hydration.
How will we denature DNA for FISH? - ANS-We treat in 70% formamide/2xSSC at 70 levels C.
How can we decorate spreading? - ANS-By dropping on wet slides and losing at a forty five
degree attitude from top to backside.
How do we hybridize DNA in FISH? - ANS-The probe is hybridized onto slides at 37 stages C
for 30mins to 16hrs depending on probe used.
How do we hydrate/rehydrate a slide? - ANS-By exposing it to diverse dilutions of ethanol
shifting from a low percent of water to a better percentage of water; one
hundred%-ninety%-80%-70%-EtOH.
How do we know if a sample is amniotic fluid or maternal urine? - ANS-Normal AF is straw
coloured and passes the Fern Test.
, How can we recognize that the slides are properly wiped clean? - ANS-They retain, in
preference to repel, water while dipped.
How do we lessen cell contamination of a chorionic villus pattern? - ANS-By casting off the
adherent maternal decidua.
How can we stimulate the increase of neoplastic cells or B-cells neoplasms? - ANS-Run one
unstimulated subculture and one with a B-cell mitogen.
How can we save a bone marrow sample if it cannot be cultured inside 4 hours? - ANS-Add to
media and store at room temp.
How can we store fixed mobile pellets? - ANS-At -20 degrees C for 2-5yrs in fixative.
How do we check for chromosome breakage syndromes? - ANS-By including clastogens.
How can we troubleshoot slides drying too speedy, without adjusting the temperature or
humidity of the thermotron? - ANS-Methanol may be delivered to the fix or bloodless slides may
be used.
How do we troubleshoot slides drying too slowly with out adjusting the temperature or humidity
of the thermotron? - ANS-Warm slides may be used or airflow across the slides may be
improved.
How do you keep away from cell contamination of prenatal specimens? - ANS-Discard the first
1-2 cc of amniotic fluid drawn.
How do you keep away from mobile infection of stable tumor specimens? - ANS-Dissect away
normal parenchyma and fatty tissue.
How do you keep away from microbial contamination? - ANS-By using aseptic approach, sterile
boxes and antibiotics/fungicides.
How do you correct a bacterial or fungal infection of a pattern? - ANS-Add antibiotic to series
media or soak/wash sample in answer containing antibiotic.
How do you accurate clotting in BM specimens? - ANS-Break up clot robotically or location in 37
degree water tub.
How do you accurate slow increase in a sample that changed into amassed in EDTA vs. Sodium
heparin? - ANS-Wash the cells before setting tradition or request every other pattern.
