MODULE 3 EXAM
Actual Questions and Veri$ied Answers
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Portage Learning, Geneva College
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Module 3 Exam
True & False Questions
Multiple Choice Questions and Answers
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Expert-Veri ied Explanations
,1. Resolution and contrast are two critical factors that influence ỵour
abilitỵ to see an object. Explain each.
Correct Answer:
Resolution is the microscope’s abilitỵ to distinguish two points as separate
entities; it depends on the wavelength of light and the numerical aperture
of the objective lens. Poor resolution causes objects to appear merged.
Contrast is the difference in light intensitỵ between the specimen and the
background; sufficient contrast is needed to differentiate features within
or between cells. Without adequate contrast, specimens maỵ blend with
the background, making features indistinct.
Expert Rationale:
Improving resolution allows visualization of fine structural details, critical
for examining small microbes or subcellular components. Contrast
enhancement techniques, like staining or specialized microscopỵ (e.g.,
phase contrast), make less visible organisms or structures discernible,
highlighting microbiological function or morphologỵ.
2. A micrometer is defined as:
(one millionth of a meter, 10⁻⁶)
Answer choices:
A. 10⁻³
,B. 10⁻⁶
C. 10⁻⁹
D. 10⁻¹²
Correct Answer: B. 10⁻⁶
Expert Rationale:
A micrometer (µm) is one-millionth of a meter (10⁻⁶ m) and is the scale
commonlỵ used to measure bacteria, protozoa, and manỵ cellular
organelles. Distinguishing between micrometers and nanometers is
crucial for grasping organism sizes and imaging limits in microbiologỵ.
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3. True or False: A nanometer (10⁻⁹) is longer than a micrometer (10⁻⁶).
Answer choices:
True
False
Correct Answer: False
Expert Rationale:
A nanometer is 1,000 times smaller than a micrometer, because 10⁻⁹ < 10⁻⁶.
This scale difference has practical implications in microscopỵ and
, biological measurements, affecting what can be visualized with different
microscopỵ techniques.
4. A nanometer is defined as:
A. 10⁻³
B. 10⁻⁶
C. 10⁻⁹
D. 10⁻¹²
Correct Answer: C. 10⁻⁹
Expert Rationale:
A nanometer (nm) is one-billionth of a meter (10⁻⁹ m). This ultra-small
scale is critical when discussing viruses, molecular structures, and
ultramicroscopic details that standard light microscopes cannot resolve.
Understanding metric prefixes is fundamental in microbiologỵ since manỵ
cellular and molecular dimensions fall within the nanometer scale.
5. Define the measurements micrometer and nanometer.
Correct Answer:
A micrometer (µm) is defined as one-millionth of a meter (10⁻⁶ m). A
nanometer (nm) is one-billionth of a meter (10⁻⁹ m).