QUESTIONS WITH DETAILED AND VERIFIED
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,topoisomerase - ANSWER - in the front of the fork and its function is to
break the hydrogen bonds and the nucleotide bases and it plays a role
in rejoining the DNA strands when replication is complete; it is able a
stabilizing enzyme that reduces the strain in the strands that occur
during replication
RNA primers - ANSWER - complementary to the parental DNA strand
RNA - ANSWER - single-stranded and contains sugar ribose and it does
NOT have a thymine but a uracil
primase - ANSWER - enzyme that facilitates the synthesis of an RNA
primer by using the unwound DNA strand as a template; it binds to the
3' end of the parent strand and begins adding complementary RNA
nucleotides as it moves toward the 5' end of the parent strand
DNA polymerase - ANSWER - enzymes that add nucleotides to the 3'
end of the existing chain via dehydration reactions
triphosphate tail - ANSWER - three phosphate group; has a negative
charge around it
, pyrophosphate - ANSWER - will undergo hydrolysis reaction which
splits the molecule into two inorganic phosphates and energy is
released in the process
leading strand - ANSWER - if nucleotides can only be added to the 3'
end of a parental strand this means that the (new) complementary RNA
primer strand is being elongated in the 5' to 3' direction. ONE RNA is
required for leading strand elongation and nucleotides are added
continuously as the replication fork progresses along the length of the
template strand.
lagging strand - ANSWER - the other template is oriented in a 3' to 5'
direction. This requires the DNA polymerase to work backward, AWAY
from the replication fork. The daughter strand that is synthesized in this
direction is called the LAGGING STRAND. The lagging strand must be
replicated in a series of fragments called Okazaki fragments rather than
in one continuous strand
DNA ligase - ANSWER - an enzyme that facilitates the joining the
Okazaki fragments
What are the 2 phases of the cell cycle? - ANSWER - Interphase and
Mitotic phase
What does interphase consist of? - ANSWER - G1, S, G2