ASCP MLS EXAM 2025 WITH ACCURATE SOLUTIONS
What feature is most often found with patients with a splenectomy on a Wright's stained blood
smear? - CORRECT -Howell-Jolly bodies
Patients with antibody to the following antigen are immune to Hepatitis B: - CORRECT
-Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous Streptococcal throat and
skin infections: - CORRECT -ASO titer and Anti-DNase B
In mass spectrometry, the "appearance potential" is defined as the: - CORRECT -
Minimum energy required for the appearance of a particular fragment ion in mass spectrum
What is generally accepted as the lower threshold value for semen pH from fertile males? -
CORRECT -7.2
Coulometry: - CORRECT -An automated method for measuring chloride which
generates silver ions in the reaction
Erythroleukemia: - CORRECT -Also known as Acute Myelogenous Leukemia type M6.
This type is associated with either the presence of both erythroid and myeloid precursors OR
strictly erythroid precursors.
Myelomonocytic leukemia - CORRECT -Is associated with an increase in myeloid and
monocytic cell precursors, but not red blood cell precursors
,Species that are Lysine (+), arginine (=) and ornithine (+): - CORRECT -Enterobacter
aerogenes, Edwardsiella species and most Serratia species
Lysine reaction for all Citrobacter: - CORRECT -Negative
Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - CORRECT -
negative reactions for the three decarboxylases commonly tested ( Lysine, arginine and
ornithine)
Which organism is MOST likely responsible for septic reactions associated with Red Blood Cell
transfusions? - CORRECT -Yersina entercolitica is most likely responsible for septic
reactions in transfusions of Red Blood Cells. This organism is usually acquired by ingestion of
contaminated food and causes mild symptoms of abdominal pain and diarrhea. Growth of Y.
entercolitica is enhanced in iron-rich environments such as red cell components.
Gaucher's disease - CORRECT -The most common lysosomal storage disease caused
by insufficient activity of the lysosomal enzyme glucocerebrosidase that leads to the deposition
of glucocerebroside in cells of the macrophage-monocyte system.
Diseases associated with dysfunctions of polymorphonuclear neutrophils (PMNs) -
CORRECT -Chediak-Higashi syndrome (CH), Chronic granulomatous disease (CGD),
Myeloperoxidase deficiency (MPO)
Nephrotic Syndrome: - CORRECT -Caused by damage to the kidneys, especially the
basement membrane of the glomerulus; which causes abnormal excretion of protein and red
blood cells in the urine. Fats are also present in the urine in most cases. A foamy appearance of
the urine is a key characteristic of this condition.
Clinical symptoms include:
Swelling
, Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: - CORRECT -The key biochemical
reactions by which the family Enterobacteriaceae can be identified include fermentation of
carbohydrates, reduction of nitrates to nitrites and the absence of cytochrome oxidase activity.
ANA - CORRECT -See 2 quiz #27
Prozone effect: - CORRECT -Results in a false positive reaction; is the result of
antibody excess and dilution of antibody can help prevent its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: - CORRECT -Minimize
the effect of interference
Key characteristics of Micrococcus: - CORRECT -Susceptible to bacitracin ("A") and
resistant to furazolidone; gram positive cocci in tetras, bacteria cells of micrococcus luteus are
larger than those of the staphylococci and can arrange in tetras
What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL, if the
urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs? - CORRECT -
95 mL/min
One method of calculating a glomerular filtration rate is using creatinine and urine volume to
determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where urine flow
rate = volume in mL /24 hours x h/60 min)
What feature is most often found with patients with a splenectomy on a Wright's stained blood
smear? - CORRECT -Howell-Jolly bodies
Patients with antibody to the following antigen are immune to Hepatitis B: - CORRECT
-Surface antigen (HbSAg)
Which two of the following tests are helpful for documenting previous Streptococcal throat and
skin infections: - CORRECT -ASO titer and Anti-DNase B
In mass spectrometry, the "appearance potential" is defined as the: - CORRECT -
Minimum energy required for the appearance of a particular fragment ion in mass spectrum
What is generally accepted as the lower threshold value for semen pH from fertile males? -
CORRECT -7.2
Coulometry: - CORRECT -An automated method for measuring chloride which
generates silver ions in the reaction
Erythroleukemia: - CORRECT -Also known as Acute Myelogenous Leukemia type M6.
This type is associated with either the presence of both erythroid and myeloid precursors OR
strictly erythroid precursors.
Myelomonocytic leukemia - CORRECT -Is associated with an increase in myeloid and
monocytic cell precursors, but not red blood cell precursors
,Species that are Lysine (+), arginine (=) and ornithine (+): - CORRECT -Enterobacter
aerogenes, Edwardsiella species and most Serratia species
Lysine reaction for all Citrobacter: - CORRECT -Negative
Key identifying characteristic for Pantoa (Enterobacter) agglomerans: - CORRECT -
negative reactions for the three decarboxylases commonly tested ( Lysine, arginine and
ornithine)
Which organism is MOST likely responsible for septic reactions associated with Red Blood Cell
transfusions? - CORRECT -Yersina entercolitica is most likely responsible for septic
reactions in transfusions of Red Blood Cells. This organism is usually acquired by ingestion of
contaminated food and causes mild symptoms of abdominal pain and diarrhea. Growth of Y.
entercolitica is enhanced in iron-rich environments such as red cell components.
Gaucher's disease - CORRECT -The most common lysosomal storage disease caused
by insufficient activity of the lysosomal enzyme glucocerebrosidase that leads to the deposition
of glucocerebroside in cells of the macrophage-monocyte system.
Diseases associated with dysfunctions of polymorphonuclear neutrophils (PMNs) -
CORRECT -Chediak-Higashi syndrome (CH), Chronic granulomatous disease (CGD),
Myeloperoxidase deficiency (MPO)
Nephrotic Syndrome: - CORRECT -Caused by damage to the kidneys, especially the
basement membrane of the glomerulus; which causes abnormal excretion of protein and red
blood cells in the urine. Fats are also present in the urine in most cases. A foamy appearance of
the urine is a key characteristic of this condition.
Clinical symptoms include:
Swelling
, Weight gain from fluid retention
Poor appetite
High blood pressure
Key Biochemical reactions for Enterobacteriaceae: - CORRECT -The key biochemical
reactions by which the family Enterobacteriaceae can be identified include fermentation of
carbohydrates, reduction of nitrates to nitrites and the absence of cytochrome oxidase activity.
ANA - CORRECT -See 2 quiz #27
Prozone effect: - CORRECT -Results in a false positive reaction; is the result of
antibody excess and dilution of antibody can help prevent its occurrence
Purpose of a bi-chromatic analyzer with dual wavelengths is: - CORRECT -Minimize
the effect of interference
Key characteristics of Micrococcus: - CORRECT -Susceptible to bacitracin ("A") and
resistant to furazolidone; gram positive cocci in tetras, bacteria cells of micrococcus luteus are
larger than those of the staphylococci and can arrange in tetras
What is the glomerular filtration rate for a patient with a serum creatinine of 2 mg/dL, if the
urine creatinine was 124 mg/dL and the urine volume was 2.2 L/24 hrs? - CORRECT -
95 mL/min
One method of calculating a glomerular filtration rate is using creatinine and urine volume to
determine creatinine clearance. The equation is as follows:
Creatinine Clearance = (urine creatinine X urine flow rate) / plasma creatinine; where urine flow
rate = volume in mL /24 hours x h/60 min)
