BIO353 Exam 2 (Mod 15-23) EXAM STUDY GUIDE WITH COMPLETE SOLUTIONS

BIO353 Exam 2 (Mod 15-23) EXAM STUDY GUIDE WITH COMPLETE SOLUTIONS True or False: It is important to be able to repeatedly fractionate the cell. - Ans:-True True or False: Homogenization buffer can be identical to an intracellular buffer. - Ans:-True After homogenization the first centrifugation pellets the ________. - Ans:-Nuclei A sucrose gradient is used to purify ___ ___ away from other ___ ___ - Ans:-plasma membrane, membrane components An organic precipitation works because the solvent ________ disrupts the ______ ___________ of the solute. - Ans:-ethanol, hydrogen bonding The binding of the signal recognition particle to the nascent peptide _____ protein synthesis. - Ans:- arrests The function of the stop- transfer and start-transfer sequences is to make a _____ protein. - Ans:- transmembrane ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 2/7 True or False: BiP acts as a chaperone - Ans:-True During vesicular transport, what is the correct sequences of events as a vesicle leaves the organelle? - Ans:-Triskelion, clathrin, recycle, Rab, SNARE The subunit of clathrin is _____ - Ans:-Triskelion The preassembled carbohydrate tree is added on ___ residue. - Ans:-Asparagine What is the role of microtubules in membrane transport? - Ans:-Transport vesicles from ER to Golgi Can COP I play a role in protein disulfide isomer position? - Ans:-Yes Does constitutive exocytosis require more signal sequences than regulative exocytosis? - Ans:-No Does the inside/outside rule apply only to regulative exocytosis? - Ans:-No True or False: The carbohydrate tree added to proteins targeted to the primary lysosome is added on an amino acid different from the amino acid where the carbohydrate tree is added to a transmembrane protein. - Ans:-False True or False: At the trans face of the Golgi, triskelion assembles into clathrin to cause removal of the putative primary lysosome. - Ans:-True Where is the number 6 carbon of mannose exposed? ©GRACEAMELIA 2024/2025 ACADEMIC YEAR. ALL RIGHTS RESERVED FIRST PUBLISH OCTOBER 2024 Page 3/7 -in the nucleus -in the rough endoplasmic reticulum -in the smooth endoplasmic reticulum -in the medial phase of the Golgi -none of the above - Ans:-None of the above Where does the receptor for mannose-6-phosphate cap the proteins destined for the primary lysosome? - Ans:-trans face of the Golgi True or False: A lipase is a protein. - Ans:-True When does the proton pump that lowers the pH below 5.5 or lower in the forming primary lysosome start working? - Ans:-after clathrin has been recycled. True or False: The nuclear lamins have no connection with the outside of the nucleus. - Ans:-False True or False: The nuclear pore complex provides a site that always lets proteins into the nucleus. - Ans:-False True or False: A half patch signal will get a protein half way into the nucleus. - Ans:-False True or False: Orphaned organelles get lipids using chaperones. - Ans:-Fals