QBM UCF Final Exam
Molecular Biology
The study, analysis, and manipulation of DNA and proteins.
Recombinant DNA Technology
The process of taking a gene from one source and joining it with another piece of DNA
and inserting that DNA into bacteria for further study and manipulation
OSHA
Occupational Health and Safety Administration
State that employers must inform employees about any potential work place hazard.
MSDS
Sheet used to describe a chemical's physical and chemical properties and any potential
hazards it may present.
CDC
Center for Disease Control
Regulates any research that involves disease-causing agents
BSL 1
Environment presents limited hazards to any researchers/employees
BSL 2
Labs that present researchers with moderate risks. Any employees must be trained and
certified to work with equipment and any hazardous chemicals need to disposed of
properly.
Most labs on UCF campus are BSL 2
BSL 3
Labs that work with pathogenic agents capable of causing disease. Require proper
ventilation
BSL 4
Labs that work with dangerous pathogens that cause fatal diseases and for which there
are no vaccines.
PPE
Personal Protective Equipment
Gloves, Labcoat, Goggles
Latex gloves are best
Note:
Never recap needles
Graduated Cylinders
Large volumes of 25 mL to 2 L
Pasteur Pipetts
Transfer pipette used when specific volumes are not required.
Micropipettes
Most accurate measuring device
Centrifuge
Separates solutions based on density. Require precise balancing.
g = Relative Centrifugal Force (universal)
Analytical Centifugation
, Sedimentation characteristics of pure samples can be analyzed.
Used to determine purity,molecular weight, size and diffusion coefficients.
Ultracentrifuge
Large-capacity Centrifugation
Used to separate large volumes (up to 2 L) of bacteria.
capable of reaching 6500 g
High-speed Centrifugation
Used for volumes of about 50 mL.
Separate microorganisms and cellular debris
Fixed-angle Rotors
Perfect for spinning pellets
Swinging-bucket Rotors
Analytical
Used for cesium chloride gradients.
Used by Messelson-Stahl to analyze DNA replication (semi-conservative)
Clear bottles
Better visualization but are less resilient to cracking and chemicals
Spectrometry
The study of radiation interacting with matter
Spectrophotometers
Measure the intensity of the wave length of light as it passes through a sample solution.
Measures absorbance
Plate Readers
Advanced spectrophotometers that can measure the absorbency of hundreds of
samples at once
Used in ELISAs, drug screenings, and enzyme assays.
Spectroscopy
The separation of light into its wave lengths
260 nm
DNA
280 nm
Proteins
595 nm
Bradford Assay
600 nm
Bacterial growth in LB agar
750 nm
Lowry Assay
Absorbance
Directly related to concentration.
A = EBc = -log(T)
(Beer's Law)
Transmittance
T=10^(-A)
Why does DNA absorb light?
Molecular Biology
The study, analysis, and manipulation of DNA and proteins.
Recombinant DNA Technology
The process of taking a gene from one source and joining it with another piece of DNA
and inserting that DNA into bacteria for further study and manipulation
OSHA
Occupational Health and Safety Administration
State that employers must inform employees about any potential work place hazard.
MSDS
Sheet used to describe a chemical's physical and chemical properties and any potential
hazards it may present.
CDC
Center for Disease Control
Regulates any research that involves disease-causing agents
BSL 1
Environment presents limited hazards to any researchers/employees
BSL 2
Labs that present researchers with moderate risks. Any employees must be trained and
certified to work with equipment and any hazardous chemicals need to disposed of
properly.
Most labs on UCF campus are BSL 2
BSL 3
Labs that work with pathogenic agents capable of causing disease. Require proper
ventilation
BSL 4
Labs that work with dangerous pathogens that cause fatal diseases and for which there
are no vaccines.
PPE
Personal Protective Equipment
Gloves, Labcoat, Goggles
Latex gloves are best
Note:
Never recap needles
Graduated Cylinders
Large volumes of 25 mL to 2 L
Pasteur Pipetts
Transfer pipette used when specific volumes are not required.
Micropipettes
Most accurate measuring device
Centrifuge
Separates solutions based on density. Require precise balancing.
g = Relative Centrifugal Force (universal)
Analytical Centifugation
, Sedimentation characteristics of pure samples can be analyzed.
Used to determine purity,molecular weight, size and diffusion coefficients.
Ultracentrifuge
Large-capacity Centrifugation
Used to separate large volumes (up to 2 L) of bacteria.
capable of reaching 6500 g
High-speed Centrifugation
Used for volumes of about 50 mL.
Separate microorganisms and cellular debris
Fixed-angle Rotors
Perfect for spinning pellets
Swinging-bucket Rotors
Analytical
Used for cesium chloride gradients.
Used by Messelson-Stahl to analyze DNA replication (semi-conservative)
Clear bottles
Better visualization but are less resilient to cracking and chemicals
Spectrometry
The study of radiation interacting with matter
Spectrophotometers
Measure the intensity of the wave length of light as it passes through a sample solution.
Measures absorbance
Plate Readers
Advanced spectrophotometers that can measure the absorbency of hundreds of
samples at once
Used in ELISAs, drug screenings, and enzyme assays.
Spectroscopy
The separation of light into its wave lengths
260 nm
DNA
280 nm
Proteins
595 nm
Bradford Assay
600 nm
Bacterial growth in LB agar
750 nm
Lowry Assay
Absorbance
Directly related to concentration.
A = EBc = -log(T)
(Beer's Law)
Transmittance
T=10^(-A)
Why does DNA absorb light?
