ELISA Worksheet -The Enzyme Linked Immunosorbent Assay

ELISA Worksheet -The Enzyme Linked Immunosorbent Assay ELISA Worksheet – Please go to Assignments to Download and submit this worksheet Enzyme-Linked Immunosorbent Assay (ELISA) uses an enzyme-linked indicator (color change) antibody to visualize antigen-antibody reactions. It is done in a microtiter plate with wells that can adsorb the reactions. It is a technique that can be used to detect antigens or antibodies in a patient’s serum, thus identifying the presence or history of infection with a microorganism. Watch the recorded lecture on the ELISA under Course Content and watch the video demonstrating the direct and indirect ELISA at to answer the following questions 1. Describe the following methods of identifying microbes or for diagnosing infections: a. Phenotypic method: b. Genotypic method: c. Immunologic method: 2. Describe the two sides to the immune system: 3. Antibodies are ________________ that function to ________________ or __________________ antigens. 4. Antibodies are found in serum. Serum is: ____________________________________________________. 5. The ELISA is an extremely sensitive test that is used to visualize ________________- ________________ reactions. 6. ELISA stands for: 7. The ELISA procedure is used to detect _______________ or _______________ in a patient’s sample. 8. Describe how the intensity of the color that develops in an ELISA is used to indicate the amount of antigen or antibody. 9. The Direct ELISA is a test for ______________________, ___________________ or ___________________ in the patient’s serum, so a known _____________________ is adsorbed to the inside of the well 10. Place the following reactants for the direct ELISA test in their proper order (Put numbers in order on the line below) 1 = enzyme-linked antibody 2 = known antibody 3 = patient serum 4 = substrate ____________________________ 11. The Indirect ELISA is a test for _______________________ in the patients serum, so a known ____________________ is adsorbed to the inside of the well 12. Place the following reactants for the indirect ELISA test in their proper order (Put numbers in order on the line below) 1 = enzyme-linked antibody 2 = known antigen 3 = patient serum 4 = substrate ____________________________ 13. If a patient test positive for antibodies in their blood, it always means they have an active infection. A) True B) False 14. In the indirect ELISA test the development of color means the patient has the antigen (Microbe part) being tested for in their serum. A) True B) False 15. The direct ELISA test can be used to test for the presence of specific autoimmune antibodies in a patient's serum. A) True B) False