LT11 Viral Vectors

Viral Vectors

 Specifically viral vectors used for animal cells – tranduced cells can be cultured,
used for nuclear transfer cloning or adult cell transplantation, gene therapy
 Replication-incapable viruses
- Deletion of essential viral components
- Use of separate vectors for packaging and viral production
- Multiple cloning site
- Selection gene(s)
- Reporter gene(s)
- Promoters – ubiquitous/tissue specific
- Enhancers (WRPE) to increase expression

Adenoviral Vector

 Non-integrating DNA virus
- Not integrated into genome and not replicated during cell
division
- Hijacks cellular transcription/translation to make viral
proteins
- Highly immunogenic as involved in human respiratory
diseases
 Usually used for in vitro protein production experiments




Retroviral Vector

 Integrating RNA viruses
- Use reverse transcription to integrate RNA into
genome