MB (ASCP) Exam 2026 \2027 LATEST VERSION WITH
COMPLETE QUESTIONS AND CORRECT DETAILED
ANSWERS \NEWEST EXAM 2026 \VERIFIED 100%
ALREADY GRADED A+
Which polymerase is used for mitochondrial replication and repair?
Gamma
What polymerase binds the 5' end to 3' end of dsDNA?
Topoisomerase
Which RNA polymerase is mainly for transcription?
RNA Pol II
Which polymerase is involved with mRNA synthesis?
RNA Pol II
What inactivates RNAses?
Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at 400C
,What type of restriction enzyme recognizes palindromic sites?
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity?
Restriction Enzyme Type II
What is necessary to make cDNA?
Reverse transcriptase
Number of SNPs in a human genome?
11 billion (10^7)
How many genes (base pairs) are in a human haploid genome?
3 billion base pairs
The polymerization of acrylamide requires:
Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration?
Inversely proportional
,What is denatured during a southern blot?
Hydrogen bonds (inter-strand bonds)
Melting temperature formula
Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe?
5' G
Proper GC content of primer
35-65%
Inhibitors of PCR
Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction:
Master mix > patient sample > positive control > negative control
Pre Area
Positive Pressure
, Post Area
Negative Pressure
Which PCR requires two steps for specificity?
Nested PCR
Which microorganism is detected by branched DNA amplification?
DNA or RNA
Which technique uses RNA, antibodies, and DNA?
Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of methylation
Bisulfite DNA
What tests are isothermal?
NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene?
AML
COMPLETE QUESTIONS AND CORRECT DETAILED
ANSWERS \NEWEST EXAM 2026 \VERIFIED 100%
ALREADY GRADED A+
Which polymerase is used for mitochondrial replication and repair?
Gamma
What polymerase binds the 5' end to 3' end of dsDNA?
Topoisomerase
Which RNA polymerase is mainly for transcription?
RNA Pol II
Which polymerase is involved with mRNA synthesis?
RNA Pol II
What inactivates RNAses?
Diethyl pyrocarbonate (DEPC) or bake glassware for 4 to 6 hours at 400C
,What type of restriction enzyme recognizes palindromic sites?
Restriction Enzyme Type II
What type of restriction enzyme lacks methylation activity?
Restriction Enzyme Type II
What is necessary to make cDNA?
Reverse transcriptase
Number of SNPs in a human genome?
11 billion (10^7)
How many genes (base pairs) are in a human haploid genome?
3 billion base pairs
The polymerization of acrylamide requires:
Catalyst (APS or TEMED) or Light activation
How does agarose gel pore size relate to concentration?
Inversely proportional
,What is denatured during a southern blot?
Hydrogen bonds (inter-strand bonds)
Melting temperature formula
Tm = (4C x GC Pairs) + (2C x AT pairs)
Which is likely to interfere with fluorescent probe?
5' G
Proper GC content of primer
35-65%
Inhibitors of PCR
Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and Bromophenol Blue
Order to add to a PCR reaction:
Master mix > patient sample > positive control > negative control
Pre Area
Positive Pressure
, Post Area
Negative Pressure
Which PCR requires two steps for specificity?
Nested PCR
Which microorganism is detected by branched DNA amplification?
DNA or RNA
Which technique uses RNA, antibodies, and DNA?
Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of methylation
Bisulfite DNA
What tests are isothermal?
NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene?
AML
