1
BCHS 3201 Lab Prelab Questions and
Answers (100% Correct Answers)
Already Graded A+
The study of all of the proteins expressed in an organism at a
particular time and/or set of conditions is called: Correct
Answer: Proteomics
© 2025 Assignment
In a microarray, to see what genes have been expressed
(transcribed) by the cell under our set of experimental
conditions, we will purify ___________ from the organism:
Guru01 - Stuvia
Correct Answer: mRNA
Expert
For our microarray project, the model organism under study
is: Correct Answer: Arabidopsis thaliana
How is the protective group removed to allow the addition of
nucleotides to the probe on the DNA chip? Correct Answer: UV
light exposure
What is in a probe pair on an Affymetrix chip? Correct
Answer: One probe to the specified gene and one probe with a
mismatch in the center of the probe to gauge non-specific
hybridization levels
Which of the following represents a properly balanced
centrifuge (check all that apply): Correct Answer: a
How will the plasmid DNA be separated from chromosomal
DNA in this plasmid purification lab? Correct Answer:
Differential centrifugation
, 2
What happens to plasmid DNA if you leave it in Solution II,
lysis buffer, for too long? Correct Answer: DNA Degrades
After lysing your E. coli cells and neutralizing the reaction, do
you keep the supernatant or pellet for the next step? Correct
Answer: Supernatant
Which has more resolving power? Correct Answer: 4% agarose
gel
© 2025 Assignment
What should you do with the liquid waste from this lab?
Correct Answer: Bleach it for 20 minutes
Guru01 - Stuvia
Why must you wait 10 minutes after adding TE/RNase to the
Expert
dry DNA pellet? Correct Answer: To allow time for the RNA to
be hydrolyzed or degraded
How will you mix the contents of the microcentrifuge tube
after adding Solution II and Solution III to the cell culture?
Correct Answer: Pipet Gently
Where should you expect to see plasmid DNA after spinning it
down out of solution? Correct Answer: smeared up one side of
the tube
What is agarose? Correct Answer: Seaweed
How do bacteria prevent their own DNA from being cleaved by
restriction enzymes? Correct Answer: By methylating their
own bacterial DNA
Which of these could be a functional restriction site? Correct
Answer: a
BCHS 3201 Lab Prelab Questions and
Answers (100% Correct Answers)
Already Graded A+
The study of all of the proteins expressed in an organism at a
particular time and/or set of conditions is called: Correct
Answer: Proteomics
© 2025 Assignment
In a microarray, to see what genes have been expressed
(transcribed) by the cell under our set of experimental
conditions, we will purify ___________ from the organism:
Guru01 - Stuvia
Correct Answer: mRNA
Expert
For our microarray project, the model organism under study
is: Correct Answer: Arabidopsis thaliana
How is the protective group removed to allow the addition of
nucleotides to the probe on the DNA chip? Correct Answer: UV
light exposure
What is in a probe pair on an Affymetrix chip? Correct
Answer: One probe to the specified gene and one probe with a
mismatch in the center of the probe to gauge non-specific
hybridization levels
Which of the following represents a properly balanced
centrifuge (check all that apply): Correct Answer: a
How will the plasmid DNA be separated from chromosomal
DNA in this plasmid purification lab? Correct Answer:
Differential centrifugation
, 2
What happens to plasmid DNA if you leave it in Solution II,
lysis buffer, for too long? Correct Answer: DNA Degrades
After lysing your E. coli cells and neutralizing the reaction, do
you keep the supernatant or pellet for the next step? Correct
Answer: Supernatant
Which has more resolving power? Correct Answer: 4% agarose
gel
© 2025 Assignment
What should you do with the liquid waste from this lab?
Correct Answer: Bleach it for 20 minutes
Guru01 - Stuvia
Why must you wait 10 minutes after adding TE/RNase to the
Expert
dry DNA pellet? Correct Answer: To allow time for the RNA to
be hydrolyzed or degraded
How will you mix the contents of the microcentrifuge tube
after adding Solution II and Solution III to the cell culture?
Correct Answer: Pipet Gently
Where should you expect to see plasmid DNA after spinning it
down out of solution? Correct Answer: smeared up one side of
the tube
What is agarose? Correct Answer: Seaweed
How do bacteria prevent their own DNA from being cleaved by
restriction enzymes? Correct Answer: By methylating their
own bacterial DNA
Which of these could be a functional restriction site? Correct
Answer: a
