MBIO 3401 Exam 1 Zhang Exam Questions and Verified Answers/Accurate Solutions| Already
Graded A+
microbes include members of which of the following groups? bacteria, archaea, fungi,
viruses
which of the following scientist-contribution pairs is not matched correctly? louis pasteur
proved spontaneous generation
what is robert kochs contribution developed the criteria for establishing a causative link
betwen an infectious agent and disease
what is leewunhoek's contribution discovered individual microbes
what is nightingales contributions founded medical statistics
kochs postulates include all of the following except what the pathogen must be shown to
contain toxins
kocks postulates a pure culture must be obtained, pathogne must be found in every
individual suffering from the disease, the isolated pathogen must be used to infect healthy
hosts, healthy individuals infected with osilated suspected pathogen must get sick with disease
who performed the first vaccination edward jenner
who discovered the first antibiotic alexander fleming
who are the ancestors of mitochondria proteobacteria
what size are normal microbes 10-100um
,what type of microbes are less than 10um prokaryotes
what is the correct order of reagents in gram stain crystal violet, idodine, ehtanol, safranin
in fluorescence micorscopy, the specimen absorbs incident light and then re-emits a _____
energy and a _____ wavelength lower, longer
which of the following microscopes allows the best view of bacterial flagella during motility
dark-field microscope
what type pf microscopy exploits differnces in refactive indices between cell parts and
surroundign media as well as all live cells phase-contrst microscopy
scanning electron microscopy specimen is fixed and embedded, it cannot be used to view
live specimen, provdes 3D image of the specimen
atomic force microscope measures van der Waals forces, example of scanning probe
microscop e
what is the best method to examining the presensce of a chemical structure with a diameter of
3nm? x-ray crystallography
what is the structure of prokaryotes that performs the same function as mitochodria in
eukaryotes cell membrane
lipids have ester linkages, while those of archaea have ether linkages
,component of peptidoglycan n-acetylmuramic acid, n-acetylglucosamine, amino acids,
peptide cross-links
prokaryotic outer membrane found in gram negative bacteria, contains porins, contains
toxic component, contains lipopolysaccharide layer
prokaryotic chromosome has a single origin of replication and then DNA is replicated
bidirectionally
what does a stalked cell in caulobacter crescentus divide to reproduce a stalked cell and a
flagellated cell
what is an extension of the cytoplasm that attaches bacteria to a surface called stalk
prokaryotic flagella driven by proton motice force, found in both gram - and gram +,
embedded in the cell envelope, used for chemotaxis
limitations for koch's postulates not all microbes can be grown in pure culture, sometimes
the causing agent can be found in both healthy and sick individuals, not all those infected
develop disease, not all pathogens have animal modes of infection
causality of koch's postulates mycobacterium tuberculosis> tuberculosis, bacillus
antracis>anthrax
association of koch's postulates men wear larger shoes and are stronger than women. do
large shoes make you stronger?
how does pasteur disprove spontaneous generation? the curve of the neck prevented
microbes from entering the broth. if the flask was broken, the broth would be contaminated.
boiled broth to get rid of exsiting microbes
, why can microbes still grow after boiling? boiling will not kill endospores, steam sterilization
and autoclaving will completely sterilize the broth
what happens if crystal violet is not added? the iodine will not react with anything and will
be able to leave lipid bilayer after adding decolorizer. once the counter stain is added, both
gram postive and negative will be red
what happens if iodine is not added during gram stain? crystal violet will not react with
anything and the decolorizer will be able to remove the CV. both gram postive and negative will
be red after safrinin
what happens if decolorizer is not added during gram stain the negative bacteria will retain
the color of the CV, and both types of bacteria will be purple
what happens if the counter stain is not added when gram staining the gram negative will
be colorless after being decoloized. gram negative will retain purple color
what type of energy do photolithoautotrophs require sunlight through photosynthesis
what type of energy do chemolithotrophs require inorganic molecule
how do you culture and isolate photolithoautotrophs incubate the culture under sun light at
room temp in a medium that does not contain organic carbon source
how do you culture and isolate a chemolithotrophs keep the culture in darkness and provide
inorganic carbon donors such as ammonium, sulfide, in the medium. do not provide organic
carbon souce
Graded A+
microbes include members of which of the following groups? bacteria, archaea, fungi,
viruses
which of the following scientist-contribution pairs is not matched correctly? louis pasteur
proved spontaneous generation
what is robert kochs contribution developed the criteria for establishing a causative link
betwen an infectious agent and disease
what is leewunhoek's contribution discovered individual microbes
what is nightingales contributions founded medical statistics
kochs postulates include all of the following except what the pathogen must be shown to
contain toxins
kocks postulates a pure culture must be obtained, pathogne must be found in every
individual suffering from the disease, the isolated pathogen must be used to infect healthy
hosts, healthy individuals infected with osilated suspected pathogen must get sick with disease
who performed the first vaccination edward jenner
who discovered the first antibiotic alexander fleming
who are the ancestors of mitochondria proteobacteria
what size are normal microbes 10-100um
,what type of microbes are less than 10um prokaryotes
what is the correct order of reagents in gram stain crystal violet, idodine, ehtanol, safranin
in fluorescence micorscopy, the specimen absorbs incident light and then re-emits a _____
energy and a _____ wavelength lower, longer
which of the following microscopes allows the best view of bacterial flagella during motility
dark-field microscope
what type pf microscopy exploits differnces in refactive indices between cell parts and
surroundign media as well as all live cells phase-contrst microscopy
scanning electron microscopy specimen is fixed and embedded, it cannot be used to view
live specimen, provdes 3D image of the specimen
atomic force microscope measures van der Waals forces, example of scanning probe
microscop e
what is the best method to examining the presensce of a chemical structure with a diameter of
3nm? x-ray crystallography
what is the structure of prokaryotes that performs the same function as mitochodria in
eukaryotes cell membrane
lipids have ester linkages, while those of archaea have ether linkages
,component of peptidoglycan n-acetylmuramic acid, n-acetylglucosamine, amino acids,
peptide cross-links
prokaryotic outer membrane found in gram negative bacteria, contains porins, contains
toxic component, contains lipopolysaccharide layer
prokaryotic chromosome has a single origin of replication and then DNA is replicated
bidirectionally
what does a stalked cell in caulobacter crescentus divide to reproduce a stalked cell and a
flagellated cell
what is an extension of the cytoplasm that attaches bacteria to a surface called stalk
prokaryotic flagella driven by proton motice force, found in both gram - and gram +,
embedded in the cell envelope, used for chemotaxis
limitations for koch's postulates not all microbes can be grown in pure culture, sometimes
the causing agent can be found in both healthy and sick individuals, not all those infected
develop disease, not all pathogens have animal modes of infection
causality of koch's postulates mycobacterium tuberculosis> tuberculosis, bacillus
antracis>anthrax
association of koch's postulates men wear larger shoes and are stronger than women. do
large shoes make you stronger?
how does pasteur disprove spontaneous generation? the curve of the neck prevented
microbes from entering the broth. if the flask was broken, the broth would be contaminated.
boiled broth to get rid of exsiting microbes
, why can microbes still grow after boiling? boiling will not kill endospores, steam sterilization
and autoclaving will completely sterilize the broth
what happens if crystal violet is not added? the iodine will not react with anything and will
be able to leave lipid bilayer after adding decolorizer. once the counter stain is added, both
gram postive and negative will be red
what happens if iodine is not added during gram stain? crystal violet will not react with
anything and the decolorizer will be able to remove the CV. both gram postive and negative will
be red after safrinin
what happens if decolorizer is not added during gram stain the negative bacteria will retain
the color of the CV, and both types of bacteria will be purple
what happens if the counter stain is not added when gram staining the gram negative will
be colorless after being decoloized. gram negative will retain purple color
what type of energy do photolithoautotrophs require sunlight through photosynthesis
what type of energy do chemolithotrophs require inorganic molecule
how do you culture and isolate photolithoautotrophs incubate the culture under sun light at
room temp in a medium that does not contain organic carbon source
how do you culture and isolate a chemolithotrophs keep the culture in darkness and provide
inorganic carbon donors such as ammonium, sulfide, in the medium. do not provide organic
carbon souce
