Jo_puenter

exto completo, conciso y actualizado, que esclarece y explica el vínculo entre la bioquímica y las bases moleculares de la salud y la enfermedad. La mejor referencia para el aprendizaje o consulta de los aspectos bioquímicos aplicados al área clínica. Presentación a todo color, con más de 700 ilustraciones. Cada capítulo inicia con un análisis titulado ‘Importancia biomédica’ y concluye con el resumen de los puntos más significativos que deben tenerse en mente. Dos capítulos nuev...

La quinta edición de Lehninger “Principios de Bioquímica”, es una introducción al tema favorito de los autores y un intento de que también lo sea del lector. En los cuatro años transcurridos desde la publicación de la tercera edición, tanto la velocidad como el alcance de los descubrimientos en bioquímica han sido extraordinarios. No sólo sabemos lo que sucede dentro de las células sino dónde y cuándo sucede, qué es lo que determina la localización y qué es lo que controla el ...

Electrophoresis through agarose or polyacrylamide gels is used to separate, analyze, identify, and purify DNA fragments. The technique is simple, rapid to perform, and capable of resolving fragments of DNA that cannot be separated adequately by other procedures, such as density gradient centrifugation

This protocol describes how to use northern hybridization to detect 15- to 150-nt small RNAs.

In this protocol, cells transfected with an Escherichia coli transposon chloramphenicol acetyltransferase (CAT) reporter plasmid are lysed by repeated cycles of freezing and thawing and cellular debris is removed by centrifugation.

Adenylation status has an important role in the regulation of mRNA metabolism: mRNAs are deadenylated before degradation, microRNAs (miRNAs) can cause deadenylation, and the poly(A) length of certain mRNAs is regulated during development. This protocol describes methods that can be used to measure the poly(A) tail length of specific mRNAs. These include, in the order of increasing sensitivity, (1) northern blotting of intact and experimentally deadenylated mRNAs and (2) northern blotting of inta...

The RNase protection assay is a sensitive method for transcription start-site localization. It begins withan RNA probe that is uniformly labeled by incorporation of one [α-32P]NTP, usually [α-32P]UTP. The RNA probe is synthesized by bacteriophage RNA polymerase (SP6, T7, or T3), which initiates transcription from specific phage promoters that have been engineered into a number of common plasmid vectors. The plasmid template contains a genomic DNA fragment spanning the region thought to contain...

The prospect of specifically controlling gene activities in vivo has become a defining hallmark of many model organisms of biological research. Where once the aim was to gain control over gene activities using endogenous control elements, new technologies have emerged that owe their remarkable specificity to heterologous components derived from evolutionarily distant species. This review highlights inducible transcriptional systems and site-specific recombination. Their quantitative and qualitat...

Química Raymond Chang 11 edición

Overlap extension polymerase chain reaction (PCR) mutagenesis can be used for the generation of a specific point mutation, insertion, or deletion within a particular DNA sequence of interest. It requires relatively little preparation compared with other mutagenesis methods and does not require the use of restriction enzymes.